卢楠,冯惠勇,王立晖

• 1:河北化工医药职业技术学院制药工程系
• 2:河北科技大学生物科学与工程学院
• 3:天津现代职业技术学院生物化工系

摘要(Abstract)：

β-糖苷酶ttβGLY是Thermus thermophilus产生的一种耐高温酶,该酶具有较高的乳糖水解活性和转糖基活性。通过对重组大肠杆菌发酵生产β-糖苷酶ttβGLY条件的研究发现,优化后的培养基组成如下:蛋白胨14g/L,酵母粉7g/L,乳清13.3g/L,NaCl 10g/L,KH2PO43g/L,K2HPO45g/L,MgSO4.7H2O 1g/L。种子培养基组成如下:蛋白胨10g/L,酵母粉5g/L,葡萄糖4g/L,NaCl 10g/L,CaCO35g/L。种龄12h,接种量8%,装量40mL/250mL三角瓶,于37℃发酵24h,菌体干重10.010g/L,酶活可达2.384U/mL,分别是优化前的4.4倍和2.4倍。

关键词(KeyWords)： 基因工程大肠杆菌;β-糖苷酶;培养基;发酵条件;优化

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 卢楠,冯惠勇,王立晖

参考文献(References)：

• [1]FENG Huiyong,JULLIEN D,HOFFMANN L,et al.Con-verting aβ-glycosidase into aβ-thransglycosidase by directedevolution[J].JBC,2005,5:234-254.
• [2]MICHEL D,LAURENT F,JEAN N L,et al.Cloning andexpression of aβ-glycosidase gene from Thermus Thermophi-lus:Sequence and biochemical characterization of the encodedenzyme[J].Glycoconjugate Journal,1999(16):27-37.
• [3]FOURAGE L,COLAS B.Synthesis ofβ-D-glucosyl-and-D-glucosyl-glucoses usingβ-glycosidase from Thermus ther-mophilus[J].Appl Microbiol Biotechnol,2001,56:406-410.
• [4]卢楠,朱文华.谢氏丙酸杆菌发酵废液中生物抑菌物质的研究[J].河北工业科技,2012,29(3):138-140.LU Nan,ZHU Wenhua.Study on biological antibacterial ma-terial in fermentation liquor of propionibacterium shermanii[J].Hebei Journal of Industrial Science and Technology,2012,29(3):138-140.
• [5]侯建革,王芳,王丽丽,等.布氏乳杆菌产生甘露醇的发酵研究[J].河北科技大学学报,2010,31(2):132-136.HOU Jiange,WANG Fang,WANG Lili,et al.Research infermentation conditions of L-actobacillus buchneri CICC 6007for product ion of mannitol[J].Journal of Hebei University ofScience and Technology,2010,31(2):132-136.
• [6]陈尧,孙国志,杨小荣,等.硅藻土助滤剂在头孢菌素C发酵液过滤中的应用研究[J].河北科技大学学报,2002,23(4):28-31.CHEN Yao,SUN Guozhi,YANG Xiaorong,et al.A study onapplication of diatomide filter aid in the filtration of cephen-lospr in C fermentation liquor[J].Journal of Hebei Universityof Science and Technology,2002,23(4):28-31.
• [7]付海云,王红权,何建勇.重组鲑鱼降钙素融合蛋白工程菌发酵工艺研究[J].河北科技大学学报,2006,27(1):54-56.FU Haiyun,WANG Hongquan,HE Jianyong.Study on fer-mentation process of fusion protein of recombinant salmon cal-citonin expressing E.coli[J].Journal of Hebei University ofScience and Technology,2006,27(1):54-56.
• [8]赵清,王葭.三孢布拉霉的菌落形态与番茄红素发酵生产能力的关系[J].河北工业科技,2009,26(4):228-230.ZHAO Qing,WANG Jia.Relationship between colonial mor-phology of blakeslea trispora and lycopene fermentation pro-duction capacity[J].Hebei Journal of Industrial Science andTechnology,2009,26(4):228-230.
• [9]张泉,郭晓宇,袁维峰,等.用于质粒DNA规模化生产的大肠杆菌发酵培养基的筛选[J].中国生物工程杂志,2006,27(1):102-105.ZHANG Quan,GUO Xiaoyu,YUAN Weifeng,et al.Selectionof liquid media for high density fermentation of recombinant E.coli for production of plasmids[J].China Biotechnology,2006,27(1):102-105.
• [10]杜木英,吴雪琴,侯大军,等.三种植物提取液的抑菌作用研究[J].四川食品与发酵,2003(3):5-8.DU Muying,WU Xueqin,HOU Dajun,et al.Studies on theinhibitio lactivities of extracts of balsampear,garlie and cap-sicum[J].Sichuan Food and Fermentation,2003(3):5-8.