透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达Cloning of vitreoscilla hemoglobin gene and expression in Rhodopseudo palustns
张立国,罗人明,周集体,吕红,杜进民,郭建博,罗晓,王晓磊
摘要(Abstract)：
构建了含有透明颤菌血红蛋白基因的可转化大肠杆菌-紫色非硫细菌穿梭质粒(pHSG-MG-vgb),将其转化到紫色非硫细菌(Rhodopseudo palustns),获得了基因工程菌EPB01。经SDS-PAGE和CO示差分光光谱分析证明,重组菌株表达了有活性的透明颤菌血红蛋白,通过低溶解氧诱导后,基因工程菌EPB01的菌体密度较对照菌增加了24%~32%。
关键词(KeyWords)： 透明颤菌血红蛋白基因;天然启动子;光合细菌;基因工程菌;低氧诱导
基金项目(Foundation):
作者(Author): 张立国,罗人明,周集体,吕红,杜进民,郭建博,罗晓,王晓磊
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