重齿鳞毛蕨中总黄酮的含量测定Content determination of total flavonoids in Dryopteris juxtaposita
杨申明,王振吉,徐成东,刘金聪
摘要(Abstract)：
黄酮类化合物具有抗癌、抗氧化、抗炎、保肝护肝、调节免疫等药理作用,一直受到国内外研究人员的高度重视。研究采用分光光度法测定了重齿鳞毛蕨(Dryopteris juxtaposita)中总黄酮的含量,并研究了提取试剂种类、试剂浓度、料液比、提取时间对总黄酮提取率的影响。结果表明采用体积分数75%乙醇加热回流180min,料液比为1g∶12mL时提取液中总黄酮提取率最高,其平均提取率为1.056%。由于此含量测定方法准确度高,操作简单,因此可作为一种高效便捷测定重齿鳞毛蕨中总黄酮含量的方法。
关键词(KeyWords)： 重齿鳞毛蕨;总黄酮;NaNO2-Al(NO3)3-NaOH;分光光度法
基金项目(Foundation): 国家自然科学基金(31260095);; 云南省应用基础计划项目(2011FZ186);云南省应用基础计划项目青年项目(2012FD049);; 楚雄师范学院基金(10YJYB02);; 云南省重点建设学科,楚雄师范学院重点建设学科基金(05YJJSXK03);; 云南省高校科技创新团队支持计划资助项目(IRTSTYN)
作者(Author): 杨申明,王振吉,徐成东,刘金聪
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