张肖建,张岩岩,高晗,孙福仁,李军山,李振江

• 1:神威药业集团有限公司
• 2:河北省中药配方颗粒工程技术研究中心

摘要(Abstract)：

为了提高牛蒡子标准汤剂的质量标准,制备了15批牛蒡子标准汤剂,通过对牛蒡苷含量及指纹图谱的研究,建立了牛蒡子标准汤剂的质量评价体系。第一煎加入8倍水,浸泡30min,煎煮20min;第二煎加入6倍水,煎煮15min,经浓缩、干燥后制得标准汤剂,应用HPLC法测定牛蒡子标准汤剂中牛蒡苷的含量及指纹图谱。结果显示:15批牛蒡子标准汤剂中牛蒡苷的转移率为21.3%～33.3%,平均转移率为27.3%,出膏率为7.1%～11.3%,平均出膏率为9.4%;通过中药指纹图谱相似度评价系统,确定了11个共有峰,通过对照品指认出了5个色谱峰,分别为绿原酸、咖啡酸、3,5-O-二咖啡酰基奎宁酸、牛蒡苷、牛蒡苷元。所建立的评价体系,牛蒡子标准汤剂制备规范,样品质量稳定,指纹图谱相似度大于0.94,对提高牛蒡子配方颗粒的质量标准具有一定的参考价值。

关键词(KeyWords)： 医药工程;牛蒡子;标准汤剂;牛蒡苷;出膏率;指纹图谱

Abstract:

Keywords:

基金项目(Foundation): 河北省科技计划项目(16272510);; 河北省重点研发计划项目(17272505D)

作者(Author): 张肖建,张岩岩,高晗,孙福仁,李军山,李振江

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015.
• [2]徐国钧.中国药材学[M].北京:中国医药科技出版社,1996.
• [3]南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006.
• [4]毕葳,雷海民,何远景,等.不同产地牛蒡子药材质量评价[J].中国中医药信息杂志,2005,12(4):47-48.BI Wei,LEI Haimin,HE Yuanjing,et al.Quality evaluation of Arctium lappa in various market[J].Chinese Journal of Information on Traditional Chinese Medicine,2005,12(4):47-48.
• [5] MARUTA Y,KAWABATA J.Antioxidative caffeoylquinic acid derivatives in the roots of burdock(Arctium lappa L.)[J].J Agric Food Chem,1995,43(10):2592-2595.
• [6]刘启迪.牛蒡子炮制工艺及质量标准研究[D].南京:南京中医药大学,2014.LIU Qidi.Study on Processing Technology and Quality Standards of Arctii Fructus[D].Nanjing:Nanjing University of Chinese Medicine,2014.
• [7]李军山,聂丽建,冀艳花,等.白鲜皮饮片标准汤剂质量评价体系构建[J].河北工业科技,2018,35(5):370-376.LI Junshan,NIE Lijian,JI Yanhua,et al.Establishment of quality evaluation system of standard decoction of Dictamni Cortex pieces[J].Hebei Journal of Industrial Science and Technology,2018,35(5):370-376.
• [8]陈士林,刘安,朱广伟,等.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.CHEN Shilin,LIU An,ZHU Guangwei,et al.Research strategies in standard decoction of medicinal slices[J].Chinese Journal of Traditional Chinese Medicine,2016,41(8):1367-1375.
• [9]朱广伟,李西文,陈士林.白芍饮片标准汤剂质量标准研究[J].世界中医药,2016,11(5):753-757.ZHU Guangwei,LI Xiwen,CHEN Shilin.Quality standard research on standard decoction of paeonialactiflora[J].World Chinese Medicine,2016,11(5):753-757.
• [10]林伟雄,乐智勇,车海燕,等.甘草饮片标准汤剂的研究[J].中国中药杂志,2017,42(3):6-11.LIN Weixiong,LE Zhiyong,CHE Haiyan,et al.Research on Glycyrrhizeae Radix standard decoction[J].Chinese Journal of Traditional Chinese Medicine,2017,42(3):6-11.
• [11]LIU H,ZHANG Y,SUN Y,et al.Determination of the major constituents in fruit of Arctium lappaL.by matrix solid-phase dispersion extraction coupled with HPLC separation and fluorescence detection[J].J Chromatogr B,2010,878(28):2707-2711.
• [12]杨申明,王振吉,徐成东,等.重齿鳞毛蕨中总黄酮的含量测定[J].河北工业科技,2014,31(1):48-52.YANG Shenming,WANG Zhenji,XU Chengdong,et al.Content determination of total flavonoids in Dryopteris juxtaposita[J].Hebei Journal of Industrial Science and Technology,2014,31(1):48-52.
• [13]姚小丽,郝亮,陈秀红.熊果苷及其中间体的含量测定[J].河北工业科技,2012,29(4):228-231.YAO Xiaoli,HAO Liang,CHEN Xiuhong.Content determination of arbutin and its intermediate[J].Hebei Journal of Industrial Science and Technology,2012,29(4):228-231.
• [14]TENG H,CHOI Y H.Optimization of ultrasonic-assisted extraction of bioactive alkaloid compounds from rhizome coptidis(CoptisChinensisFranch.)usingresponsesurface methodology[J].Food Chem,2014,142:299-305.
• [15]张肖建,霍保军,李军山,等.葛根水煎剂和配方颗粒HPLC指纹图谱相关性研究[J].河北工业科技,2018,35(3):226-230.ZHANG Xiaojian,HUO Baojun,LI Junshan,et al.Correlation of fingerprint of puerariae water decoction and formula granules by HPLC[J].Hebei Journal of Industrial Science and Technology,2018,35(3):226-230.
• [16]宾婕,施建莲,胡珊珊,等.不同产地牛蒡子的指纹图谱研究[J].药物资讯,2017,6(5):121-126.BIN Jie,SHI Jianlian,HU Shanshan,et al.Identification of Arctium lappa L.from different cultivation places by HPLC fingerprint[J].Pharmacy Information,2017,6(5):121-126.